Eagle’s MEM|NISSUI培养基|金畔生物

Eagle’s MEM

货号:AYJ0041A
包装:100g,500g

  • 品牌:NISSUI
  • 外观:粉末状培养基
  • 产品详情质检信息

    Directions
    Dissolve 9.4g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121 centigrade for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate solution. (pH will be 7.1-7.4 at 37 centigrade in an
    atmosphere of 5% CO2 in air by adding 12.5-22.0mL of sodium bicarbonate solution) This mixture should be stopper tightly and stored in a cool, dark
    place. At the time of use, aseptically add 0.292 g of L-glutamine (sterilized) and the desired amount of serum.

    Summary
    Eagle’s MEM “Nissui” 1 is a powdered medium prepared according to prescription published by Harry Eagle in 1959. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization was performed by filtration using a membrane filter. Nissui Pharmaceutical Co., Ltd. has succeeded in preparing an autoclavable powder medium. This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired. This medium has a growth-promoting effect for cells such as HeLa or L and other cell lines.
    Storage and Expiration
    Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
    Precautions
    Do not use the product after its expiry date. Quality of the product is not warranted after its shelf life.
    When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician.
    Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.

    Formula


    ComponenComponents
    In 9.4 g/L
    Eagle’s MEM|NISSUI培养基|金畔生物
    Sodium Chloride
    6,800.0 mg
    Potassium Chloride
    400.0 mg
    Calcium Chloride
    200.0 mg
    Magnesium Sulfate
    93.5 mg
    Sodium Dihydrogen Phosphate
    115.0 mg
    Glucose
    1,000.0 mg
    L-Arginine Hydrochloride
    126.0 mg
    L-Cystine Dihydrochloride, H2O
    31.4 mg
    L-Tyrosine
    36.0 mg
    L-Histidine Hydrochloride, H2O
    42.0 mg
    L-Isoleucine
    52.0 mg
    L-Leucine
    52.0 mg
    L-Lysine Hydrochloride
    73.0 mg
    L-Methionine
    15.0 mg
    L-Phenylalanine
    32.0 mg
    L-Threonine
    48.0 mg
    L-Tryptophan
    10.0 mg
    L-Valine
    46.0 mg
    Succinic Acid
    75.0 mg
    Sodium Succinate, 6H2O
    100.0 mg
    Choline Bitartrate
    1.8 mg
    Folic Acid
    1.0 mg
    Inositol
    2.0 mg
    Nicotinamide
    1.0 mg
    Calcium Pantothenate
    1.0 mg
    Pyridoxal Hydrochloride
    1.0 mg
    Riboflavin
    0.1 mg
    Thiamin Hydrochloride
    1.0 mg
    Biotin
    0.02 mg
    Kanamycin
    60.0 mg (Titer)
    Phenol Red
    6.0 mg