RPMI 1640 Medium|NISSUI培养基|金畔生物

RPMI 1640 Medium

货号:AYJ0095A-100g
包装:100g

  • 品牌:NISSUI
  • 产品详情质检信息

    Directions
    Dissolve 10.2 g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121°C for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate. (pH will be 7.1-7.4 at 37°C, in an atmosphere of 5% CO2 in air by adding 18-30 mL of sodium bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.

    Summary
    RPMI 1640 is a medium for suspension culture investigated by George E. Moore, former director of Roswell Park Memorial Institute. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to overcome the difficulties associated with filtersterilized media, such as contamination by Mycoplasma. This medium has growth promoting effect to primary cultures of mouse and human leukemia cells, various kinds of cell lines and KATO-III cells.
    Storage and Expiration
    Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
    Precautions
    Do not use the product after its expiry date.
    Quality of the product is not warranted after its shelf life.
    When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician. Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.

    Formula


    Components
    In 10.2 g/L
    L-Arginine Hydrochloride
    240.0 mg
    L-Asparagine, H2O
    56.8 mg
    L-Asparatic Acid
    20.0 mg
    L-Cysteine Hydrochloride, H2O
    72.9 mg
    L-Glutamic Acid
    20.0 mg
    Succinic Acid
    46.0 mg
    Sodium Succinate,6 H2O
    164.0 mg
    Glutathione
    1.0 mg
    Glycine
    10.0 mg
    L-Histidine Hydrochloride, H2O
    20.3 mg
    L-Hydroxyproline
    20.0 mg
    L-Isoleucine
    50.0 mg
    L-Leucine
    50.0 mg
    L-Lysine Hydrochloride
    40.0 mg
    L-Methionine
    15.0 mg
    L-Phenylalanine
    15.0 mg
    L-Proline
    20.0 mg
    L-Serine
    30.0 mg
    L-Threonine
    20.0 mg
    L-Tryptophan
    5.0 mg
    L-Tyrosine
    20.0 mg
    L-Valine
    20.0 mg
    Biotin
    0.2 mg
    Calcium Pantothenate
    0.25 mg
    Choline Chloride
    3.0 mg
    Folic Acid
    1.0 mg
    Inositol
    35.0 mg
    Nicotinamide
    1.0 mg
    p-Aminobenzoic Acid 
    1.0 mg
    Pyridoxine Hydrochloride
    1.0 mg
    Riboflavin
    0.2 mg
    Thiamin Hydrochloride
    1.0 mg
    Cyanocobalamin
    0.005 mg
    Sodium Chloride
    6,000.0 mg
    Potassium Chloride
    400.0 mg
    Calcium Nitrate
    69.5 mg
    Monosodium Phosphate
    677.0 mg
    Magnesium Sulfate
    48.8 mg
    Glucose
    2,000.0 mg
    Phenol Red
    5.0 mg