kit – 爱尔兰Megazym酶试剂盒 http://www.streck.net.cn 美国Dyets纯化饲料模型饲料中国代理商金畔官网 Mon, 07 Oct 2024 04:15:03 +0000 zh-CN hourly 1 https://wordpress.org/?v=6.2.6 Megazyme 淀粉总量检测试剂盒 K-TSTA-100A http://www.streck.net.cn/1931.html Sat, 09 Dec 2017 07:05:51 +0000 http://www.jinpanbio.cn/?p=1931 继续阅读 →]]> 淀粉总量检测试剂盒

英文名：Total Starch (AA/AMG) Assay Kit

货号：K-TSTA-100A

规格：100 assays per kit

市场价： 3824元

分析物意义：主要的食品组分

Megazyme检测试剂盒优点：选择用GOPD试剂或己糖激酶或6-磷酸葡萄糖脱氢酶测定D-葡萄糖的快速检测试剂盒

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 

]]> 抗性淀粉检测试剂盒 K-RSTAR Resistant Starch Assay Kit http://www.streck.net.cn/1924.html Sat, 09 Dec 2017 06:31:06 +0000 http://www.jinpanbio.cn/?p=1924 继续阅读 →]]> 抗性淀粉检测试剂盒

英文名：Resistant Starch Assay Kit

货号：K-RSTAR

规格：100 assays per kit

市场价： 3472元

The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.

Colourimetric method for the determination of Resistant Starch
in cereal products and feeds

Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose +
maltose

(3) Dissolution of resistant starch pellet in KOH and neutralisation

(α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H2O → D-glucose

(glucose oxidase)
(5) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 120 min (plus overnight incubation)
Detection limit: 2-100% of sample weight
Application examples:
Plant materials, starch samples and other materials
Method recognition:
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Measures enzyme resistant starch
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

FAQ解答

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

 

]]> 膳食纤维总量检测试剂盒 K-TDFR-200A Total Dietary Fibre Assay Kit http://www.streck.net.cn/1920.html Sat, 09 Dec 2017 06:18:48 +0000 http://www.jinpanbio.cn/?p=1920 继续阅读 →]]> 膳食纤维总量检测试剂盒

英文名：Total Dietary Fibre Assay Kit

货号：K-TDFR-200A

规格：200 assays per kit

市场价： 4736元

分析物意义：小肠不能消化的碳水化合物

Megazyme检测试剂盒优点：试剂稳定。成本低。AOAC方法985.29和991.43；AACC方法32-07和32-21

The Total Dietary Fiber test kit is suitable is suitable for the measurement and analysis of Total Dietary Fiber.

For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials

Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol
precipitation

(4) Ash and residual protein determined on DF residues
and subtracted

Kit size: 100 / 200 assays
Method: Hydrolysis / removal of non-dietary
fibre components
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:
AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)

Total Dietary Fiber Assay Kit, for the measurement and analysis of total, soluble and insoluble dietary fiber according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.

2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.

3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

Two separate methods are described in the associated data booklet:

METHOD 1:

DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER

Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

METHOD 2:

DETERMINATION OF TOTAL DIETARY FIBER

Based on AACC method 32-05.01 and AOAC Method 985.29.

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years
  • High purity / standardised enzymes employed
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format

 

 

]]>