Megazyme 半乳甘露聚糖检测试剂盒(K-GALM)

特色

 

半乳甘露聚糖检测试剂盒

货号:K-GALM
包装:1 kit

 

  • 英文名:Galactomannan Assay Kit
  • 品牌:Megazyme

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半乳甘露聚糖检测试剂盒,Galactomannan Assay Kit, 货号:K-GALM,100 次检测,适于食品和植物产品中办乳甘露聚糖的检测和分析。

产品详情

半乳甘露聚糖检测试剂盒, Galactomannan Assay Kit, 货号:K-GALM
品牌:Megazyme
货号:K-GALM
中文品名:半乳甘露聚糖检测试剂盒
品名:Galactomannan Assay Kit
规格:100 次检测
应用:半乳甘露聚糖检测试剂盒适于食品和植物产品中办乳甘露聚糖的检测和分析。
原理:
Megazyme 半乳甘露聚糖检测试剂盒(K-GALM)
检测方法:分光光度法 @340 nm
反应时间:~90min
检测限:样品重量的1-100%
应用案例:
种子,碾磨粉和食品配料
方法识别:
新方法
试剂盒组成:
Bottle 1: 缓冲溶液(25 mL, pH 8.6) 添加叠氮钠(0.02%)作为防腐剂(缓冲液A)
4°C下稳定超过 2年
Bottle 2: NAD+
-20°C稳定超过 5年
Bottle 3: β-甘露聚糖酶悬浮液(A. niger; 1.1 mL)
4°C下稳定超过 4年
Bottle 4: β-半乳糖苷酶(瓜尔豆胶种子)加上β-甘露聚糖酶悬浮液,2.2 mL。
4°C下稳定超过 4年
Bottle 5: β-半乳糖脱氢酶 2.4 mL。
4°C下稳定超过 2年
Bottle 6: D-半乳糖标准溶液 (5 mL, 0.4 mg/mL 溶于0.02%叠氮钠溶液中)。
4°C下稳定超过 4年
Bottle 7: 长豆角半乳糖甘露聚糖粉质控品(半乳甘露聚糖含量在小瓶标签上标明)
室温下稳定超过 5年

优点:

试剂盒内包含半乳糖脱氢酶
价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年
仅提供酶试剂盒
操作简单
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
适合手工、微孔板和自动分析仪分析。
参考文献:
An enzymic technique for the quantitation of galactomannan in guar Seeds. McCleary, B. V. (1981). Lebensmittel-Wissenschaft & Technologie, 14, 56-59.
Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
Metabolic and genetic perturbations accompany the modification of galactomannan in seeds of Medicago truncatula expressing mannan synthase from guar (Cyamopsis tetragonoloba L.). Naoumkina, M., Vaghchhipawala, S., Tang, Y., Ben, Y., Powell, R. J. & Dixon, R. A. (2008). Plant Biotechnology Journal, 6(6), 619-631.
Enzymatic improvement of guar‐based thickener for better‐quality silk screen printing. Baldaro, E., Gallucci, M., Formantici, C., Issi, L., Cheroni, S. & Galante, Y. M. (2012). Coloration Technology, 128(4), 315-322.
Nutrient utilisation and intestinal fermentation are differentially affected by the consumption of resistant starch varieties and conventional fibres in pigs. Rideout, T. C., Liu, Q., Wood, P. & Fan, M. Z. (2008). British Journal of Nutrition, 99(05), 984-992.
使用手册:
K-GALM使用手册
相关产品:
Galactomannan (Guar; Medium Viscosity)
Galactomannan (Carob; Low Viscosity)
Galactomannan (Carob; High Viscosity)
Galactomannan (Guar; High Viscosity)
Galactomannan (Guar; High Visc, Gal depleted; 21% Gal)
Galactomannan (Guar; High Visc, Gal depleted; 28% Gal)
endo-1,4 β-Mannanase (Bacillus sp.)

总膳食纤维检测试剂盒, Total Dietary Fiber Assay Kit, 货号:K-TDFR, 100/200次, 适合分析和检测总膳食纤维(可溶性膳食纤维 + 不溶性膳食纤维)含量。

特色

总膳食纤维检测试剂盒, Total Dietary Fiber Assay Kit, 货号:K-TDFR
品牌:Megazyme
货号:K-TDFR
中文品名:总膳食纤维检测试剂盒
品名:Total Dietary Fiber Assay Kit
规格:100/200次
应用:适合分析和检测总膳食纤维(可溶性膳食纤维 + 不溶性膳食纤维)含量。
原理:
谷物制品、食品、饲料和其他材料中总膳食纤维的测定
纤维是一种复杂的有机物质混合物,包括亲水性化合物,如可溶性和不溶性多糖,不可消化的低聚糖,以及一系列不膨胀又或多或少疏水的化合物,如角质素、木栓素和木质素。
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose
(protease)
(2) Protein + H2O → peptides
(3) Dietary fiber determined gravimetrically following alcohol precipitation
(4) Ash and residual protein determined on DF residues and subtracted
检测方法:水解/去除非膳食纤维成分
检测时间:~ 100 min
检测限:样品重量的0.5-100%
方法1:总、可溶性和不溶性膳食纤维的测定
根据AOAC方法991.43 “食品中总, 可溶性和不溶性膳食纤维” (First Action 1991) 和AACC方法 32-07.01 “食物和食品中总, 可溶性和不溶性膳食纤维含量确定” (Final Approval 10-16-91).
方法2:总膳食纤维的测定
根据AACC方法32-05.01方法AOAC方法985.29.
方法识别:
AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
AACC (Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01)
CODEX (Type I Method)
应用案例:
食品配料、食品成品和其它食材。
产品列表:
货号 品名 规格
K-TDFR-100A Total Dietary Fiber Assay Kit 100 assays
K-TDFR-200A Total Dietary Fiber Assay Kit 200 assays
试剂盒组成:

100次 总膳食纤维检测试剂盒 (货号 K-TDFR-100A)

Bottle 1: 热稳定的α-淀粉酶(10 mL, ~ 3,000 U/mL (Ceralpha方法); ~ 10,000 U/mL 对于水溶性淀粉) (Megazyme 货号 E-BLAAM)
Bottle 2: 纯化蛋白酶 (10 mL, 50 mg/mL; ~ 350 酪氨酸 U/mL) (Megazyme 货号 E-BSPRT)
Bottle 3: 纯化淀粉葡萄糖苷酶 (20 mL, 3,300 U/mL 对于水溶性淀粉) (Megazyme 货号 E-AMGDF)

200次 总膳食纤维检测试剂盒 (货号 K-TDFR-200A)

Bottle 1: 热稳定的α-淀粉酶(10 mL, ~ 3,000 U/mL (Ceralpha方法); ~ 10,000 U/mL 对于水溶性淀粉) (Megazyme 货号 E-BLAAM)
Bottle 2: 纯化蛋白酶 (10 mL, 50 mg/mL; ~ 350 酪氨酸 U/mL) (Megazyme 货号 E-BSPRT)
Bottle 3:(x 2) 纯化淀粉葡萄糖苷酶 (20 mL, 3,300 U/mL 对于水溶性淀粉) (Megazyme 货号 E-AMGDF)

优点:

价格极具竞争力
所有试剂可稳定保存2年以上
使用高纯度标准化的酶
官网上提供Mega-Calc™软件工具用于有序的原始数据处理
形式简单
参考文献:
Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
使用手册:
K-TDFR使用手册
相关产品:
Total Dietary Fiber Controls
Available Carbohydrates/Dietary Fiber Assay Kit
Amyloglucosidase (Aspergillus niger)
α-Amylase (Bacillus licheniformis)
Protease Subtilisin A (from Bacillus licheniformis) Powder
Protease (Subtilisin A from Bacillus licheniformis)
Amyloglucosidase (Aspergillus niger) Glycerol Free
Amyloglucosidase (Aspergillus niger) Powder
Ambersep 200 H+ Ion Exchange Resin
Amberlite FPA OH- Ion Exchange Resin
Celite

Megazyme果聚糖检测试剂盒, Fructan Assay Kit, 货号:K-FRUC, 适于植物提取物和含有淀粉、蔗糖和其他糖的食物中果聚糖的特定检测和分析。

特色

果聚糖检测试剂盒, Fructan Assay Kit, 货号:K-FRUC
品牌:Megazyme
货号:K-GLUC
中文品名:果聚糖检测试剂盒
品名:Fructan Assay Kit
规格:100次/盒
应用:果聚糖检测试剂盒适于植物提取物和含有淀粉、蔗糖和其他糖的食物中果聚糖的特定检测和分析。
原理:
Megazyme果聚糖检测试剂盒, Fructan Assay Kit, 货号:K-FRUC, 适于植物提取物和含有淀粉、蔗糖和其他糖的食物中果聚糖的特定检测和分析。
检测方法:分光光度法 @410 nm
反应时间:~90min
检测限:样品重量的1-100%
应用案例:
面粉、植物食材(例如,洋葱)、食品和其它食材
方法识别:
AOAC (方法 999.03), AACC (方法 32-32.01) 和 CODEX (Type III 方法)
试剂盒组成:
Bottle 1: 蔗糖酶,加上β-淀粉酶、支链淀粉酶、麦芽糖酶,冷干粉。
-20°C稳定超过 5年
Bottle 2: 果聚糖酶。重组菊粉外切酶和重组菊粉内切酶,冻干粉。
-20°C稳定超过 5年
Bottle 3: 果聚糖受控粉。伴有α-纤维素的果聚糖冻干粉。
室温下干燥保存稳定超过 5年
Bottle 4: 蔗糖受控粉。伴有α-纤维素的蔗糖冻干粉。
室温下干燥保存稳定超过 5年
Bottle 5: D-葡萄糖标准溶液(5 mL, 1.0 mg/mL) 溶于 0.2% (w/v)苯甲酸溶液中。
室温下稳定超过 5年

优点:

所有试剂制备后可保质超过12个月
价格非常具有竞争力
不受高浓度蔗糖/还原糖影响
仅Megazyme能提供果聚糖检测试剂盒
检测方法十分简单
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
参考文献:
Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
Measurement of total fructan in foods by enzymatic/spectrophotometric method: Collaborative study. McCleary, B. V., Murphy, A. & Mugford, D. C. (2000). Journal of AOAC International, 83(2), 356-364.
Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
Physical, microscopic and chemical characterisation of industrial rye and wheat brans from the Nordic countries. Kamal-Eldin, A., L?rke, H. N., Knudsen, K. E. B., Lampi, A. M., Piironen, V., Adlercreutz, H., Katina, K., Poutanen, K. & Aman, P. (2009). Food & Nutrition Research, 53.
Waxy endosperm accompanies increased fat and saccharide contents in bread wheat (Triticum aestivum) grain. Yasui, T. & Ashida, K. (2011). Journal of Cereal Science, 53(1), 104-111.
Characterization and in vitro immunomodulatory screening of fructo-oligosaccharides of Asparagus racemosus Willd. Thakur, M., Connellan, P., Deseo, M. A., Morris, C., Praznik, W., Loeppert, R. & Dixit, V. K. (2012). International Journal of Biological Macromolecules, 50(1), 77-81.
Steam‐girdling of barley (Hordeum vulgare) leaves leads to carbohydrate accumulation and accelerated leaf senescence, facilitating transcriptomic analysis of senescence‐associated genes. Parrott, D. L., McInnerney, K., Feller, U. & Fischer, A. M. (2007). New Phytologist, 176(1), 56-69.
Contents of dietary fibre components and their relation to associated bioactive components in whole grain wheat samples from the HEALTHGRAIN diversity Screen. Andersson, A. A. M., Andersson, R., Piironen, V., Lampi, A. M., Nystr?m, L., Boros, D., Fra?, A., Gebruers, K., Courtin, C. M., Delcour, J. A., Rakszegi, M., Bedo, Z., Ward, J. L., Shewry, P. R. & man, P. (2013). Food Chemistry, 136(3-4), 1243-1248.
Distribution and characterisation of fructan in wheat milling fractions. Hask?, L., Nyman, M. & Andersson, R. (2008). Journal of Cereal Science, 48(3), 768-774.
Comparison of a colorimetric and a high‐performance liquid chromatography method for the determination of fructan in pasture grasses for horses. Longland, A. C., Dhanoa, M. S. & Harris, P. A. (2012). Journal of the Science of Food and Agriculture, 92(9), 1878-1885.
Relationship of Grain Fructan Content to Degree of Polymerisation in Different Barleys. Nemeth, C., Andersson, A. A. M., Andersson, R., Mangelsen, E., Sun, C. & ?man, P. (2014). Food and Nutrition Sciences, 2014, 5(6), 581-589.
Chain length of inulin affects its degradation and the microbiota in the gastrointestinal tract of weaned piglets after a short-term dietary application. Pa?lack, N., Al-Samman, M., Vahjen, W., M?nner, K. & Zentek, J. (2012). Livestock Science, 149(1-2), 128-136.
How does the preparation of rye porridge affect molecular weight distribution of extractable dietary fibers?Rakha, A., ?man, P. & Andersson, R. (2011). International Journal of Molecular Sciences, 12(5), 3381-3393.
使用手册:
K-FRUC使用手册
相关产品:
Fructan HK Assay Kit
Azo-Fructan
Azo-Fructan
Inulin
Fructooligosaccharides (FOS)

Megazyme D-甘露糖/D-果糖/D-葡萄糖检测试剂盒,D-Mannose/D-Fructose/D-Glucose Assay kit (K-MANGL),用于植物产品和多糖酸水解产物中D-甘露糖、D-果糖和D-葡萄糖特定检测和分析。

特色

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒, D-Mannose/D-Fructose/D-Glucose Assay kit, 货号:K-MANGL
品牌:Megazyme
货号:K-MANGL
中文品名:D-甘露糖/D-果糖/D-葡萄糖检测试剂盒
品名:D-Mannose/D-Fructose/D-Glucose assay kit
规格:55 次检测
应用:D-甘露糖/D-果糖/D-葡萄糖检测试剂盒,用于植物产品和多糖酸水解产物中D-甘露糖、D-果糖和D-葡萄糖特定检测和分析。
原理:
Megazyme D-甘露糖/D-果糖/D-葡萄糖检测试剂盒,D-Mannose/D-Fructose/D-Glucose Assay kit (K-MANGL),用于植物产品和多糖酸水解产物中D-甘露糖、D-果糖和D-葡萄糖特定检测和分析。
检测方法:分光光度法 @340 nm
反应时间:~30min
检测限:0.7 mg/L
应用案例:
食品,酵母细胞制备,酶解产物和其他原料(如生物培养、样品等)
方法识别:
新方法
试剂盒组成:
Bottle 1: 缓冲溶液(12 mL, pH 7.6) 添加叠氮钠(0.02%)作为防腐剂
4°C下稳定超过 2年
Bottle 2: NADP+, ATP。
-20°C稳定超过 5年
Bottle 3: 己糖激酶和葡萄糖-6-磷酸脱氢酶悬浮液, 1.2mL.
4°C下稳定超过 2年
Bottle 4: 磷酸葡萄糖异构酶悬浮液(1.2 mL)。
4°C下稳定超过 2年
Bottle 5: 磷酸甘露糖异构酶悬浮液 (1.2 mL)。
4°C下稳定超过 2年
Bottle 6: D-甘露糖、D-果糖和D-葡萄糖标准溶液(5 mL, 每种糖浓度为0.1 mg/mL)。
4°C下稳定超过 2年

优点:

价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年(针对手工分析应用)
仅提供酶试剂盒
操作简单
快速反应、检测迅速
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
参考文献:
Altered physiology and biochemistry of imported litchi fruit held under different vapor pressure deficits. Somboonkaew, N. & Terry, L. A. (2010). Journal of Agricultural and Food Chemistry, 58(10), 6209-6218.
A new bacterial hydrolase specific for the compatible solutes α-D-mannopyranosyl-(1→2)-D-glycerate and α-D-glucopyranosyl-(1→2)-D-glycerate. Alarico, S., Empadinhas, N. & da Costa, M. S. (2013). Enzyme and Microbial Technology, 52(2), 77-83.
The molecular characterization of a novel GH38 α-mannosidase from the crenarchaeon Sulfolobus solfataricus revealed its ability in de-mannosylating glycoproteins. Cobucci-Ponzano, B., Conte, F., Strazzulli, A., Capasso, C., Fiume, I., Pocsfalvi, G., Rossi, M. & Moracci, M. (2010). Biochimie, 92(12), 1895-1907.
The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate.Nobre, A., Empadinhas, N., Nobre, M. F., Lourenço, E. C., Maycock, C., Ventura, M. R., Mingote A. & da Costa, M. S. (2013). Planta, 237(3), 891-901.
使用手册:
K-MANGL使用手册
相关产品:
D-Glucose HK Assay Kit
D-Fructose/D-Glucose Assay Kit
Maltose/Sucrose/D-Glucose Assay Kit
Sucrose/D-Fructose/D-Glucose Assay Kit

Megazyme 昆布六糖, Laminarihexaose, 货号:O-LAM6, 30mg, 用于研究、酶生化分析和体外诊断分析。

特色

昆布六糖, Laminarihexaose, 货号:O-LAM6
品牌:Megazyme
货号:O-LAM6
中文品名:昆布六糖
品名:Laminarihexaose
CASN:29842-30-6
纯度: >95%
包装: 30 mg
来源:通过控制凝胶多糖的酸性水解制得。
用途:高纯度昆布六糖用于研究、酶生化分析和体外诊断分析。
Megazyme 昆布六糖, Laminarihexaose, 货号:O-LAM6, 30mg, 用于研究、酶生化分析和体外诊断分析。
参考文献:
Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. Pedersen, H. L., Fangel, J. U., McCleary, B., Ruzanski, C., Rydahl, M. G., Ralet, M. C., Farkas, V., Von Schantz, L., Marcus, S. E., Andersen, M.C. F., Field, R., Ohlin, M., Knox, J. P., Clausen, M. H. & Willats, W. G. T. (2012). Journal of Biological Chemistry, 287(47), 39429-39438.
Purification and Characterization of a Thermostable Laminarinase from Penicillium rolfsii c3-2 (1) IBRL. Lee, K. C., Arai, T., Ibrahim, D., Kosugi, A., Prawitwong, P., Lan, D., Murata, Y. & Mori, Y. (2014). BioResources, 9(1), 1072-1084
Family 6 carbohydrate binding modules recognize the non-reducing end of β-1,3-linked glucans by presenting a unique ligand binding surface. van Bueren, A. L., Morland, C., Gilbert, H. J. & Boraston, A. B. (2005). Journal of Biological Chemistry, 280(1), 530-537.
Structural characterization of neutral oligosaccharides by laser-enhanced in-source decay of MALDI-FTICR MS. Yang, H., Yu, Y., Song, F. & Liu, S. (2011). Journal of The American Society for Mass Spectrometry, 22(5), 845-855.
Isoliquiritigenin (4,2′,4′-trihydroxychalcone): A new matrix-assisted laser desorption/ionization matrix with outstanding properties for the analysis of neutral oligosaccharides. Yang, H., Wang, J., Song, F., Zhou, Y. & Liu, S. (2011). Analytica Chimica Acta, 701(1), 45-51.
An olive pollen protein with allergenic activity, Ole e 10, defines a novel family of carbohydrate-binding modules and is potentially implicated in pollen germination. Barral, P., Suarez, C., Batanero, E., Alfonso, C., de Dios Alche, J., Rodriguez-Garcia, M. I., Villalba, M., Rivas, G. & Rodriguez, R. (2005). Biochem. J, 390, 77-84.
Gas-phase fragmentation of oligosaccharides in MALDI laser-enhanced in-source decay induced by thermal hydrogen radicals. Yang, H., Li, M., Li, Z. & Liu, S. (2012). Analyst, 137(16), 3624-3626.
使用手册:
O-LAM6使用手册
相关产品:
Laminaritriose
Laminaritriose
Laminaritetraose
Laminaripentaose
Laminarihexaose
4-Nitrophenyl-β-laminaritetraoside
4-Methylumbelliferyl-β-laminaritetraoside
1,3-Beta-Glucazyme HS Tablets
1,3-β-Glucazyme Tablets
AZCL-Pachyman
AZCL-Curdlan
Curdlan
Pachyman (1,3-β-D-Glucan)

甘油检测试剂盒 K-GCROL 70 assays (manual) / 700 assays (microplate)

特色

甘油检测试剂盒
英文名:Glycerol Assay Kit
货号:K-GCROL
规格:70 assays (manual) / 700 assays (microplate)
市场价: 1808元

分析物意义:常见食品组分,或作为甜味剂,或用于改善口感

Megazyme检测试剂盒优点:新型的药片模式,性质更稳定,反应快

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+

Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK

Advantages

  • Novel tablet format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual and microplate formats

 

Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q2. Is K-GCROL specific for glycerol?

K-GCROL is highly specific for glycerol.
Some compounds that are known not to react or interfere with the assay include:
Polyethylene glycol
Ethylene glycol
Propylene glycol

Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q4. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q5. Is the Glycerol Assay Kit (K-GCROL) suitable for measurement using cell culture media samples?

Yes, assuming that the concentration of the analyte in the sample (after sample preparation) is above the limit of detection for the kit. It may be sufficient to use the sample directly in the assay after clarification by centrifugation / filtering followed, by dilution (if required) in distilled water.

Q6. Can the test kit be used to measure biological fluids and what sample preparation method should be used?

The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2

The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.

Q7. Can the manual assay format be scaled down to a 96-well microplate format?

The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.

There a 3 main methods for calculation of results using the microplate format:

  1. The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
  2. Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
  3. Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.

Q8. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q9. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q10. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

Q11. I have some doubts about the appearance/quality of a kit component what should be done?

If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).

Q12. How much sample should be used for the clarification/extraction of my sample?

The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).

Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.

Q13. Can the sensitivity of the kit assay be increased?

For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.

Q14. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?

The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.

However, the level of accuracy is obviously analyst and sample dependent.

Q15. Is it possible to add a larger volume then 2 μL of enzyme to the microplate assay? In some instances 2 μL can be difficult to pipette manually.

Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.

Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.

Q16. To measure fermentation samples contain high microbial cell density, is cell disruption required?

Cell disruption is only require to measure glycerol within microbial cells.

To measure glycerol in the extracellular media only then cell disruption is not required and centrifugation of the sample may be sufficient, e.g.:

(a) Determination of glycerol in cell culture media/supernatants. In general, the concentration of glycerol in cell culture media/supernatants can be determined without any sample treatment (except clarification by centrifugation/filtering or dilution according to the dilution table, if necessary). Typically, no clarification or dilution is required, and a sample volume of 0.1 mL is satisfactory.

If interference is suspected then sample clarification/deproteinisation using carrez reagents or perchloric acid should be used (methods are provided in the kit booklet).

Megazyme 淀粉总量检测试剂盒 K-TSTA-100A

特色

淀粉总量检测试剂盒

英文名:Total Starch (AA/AMG) Assay Kit

货号:K-TSTA-100A

规格:100 assays per kit

市场价: 3824

分析物意义:主要的食品组分

Megazyme检测试剂盒优点:选择用GOPD试剂或己糖激酶或6-磷酸葡萄糖脱氢酶测定D-葡萄糖的快速检测试剂盒

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 

MEGAZYME 淀粉总量HK检测试剂盒 K-TSHK

特色

Megazyme中文站-经销爱尔兰MEGAZYME试剂盒、酶标准品、糖酶片剂

淀粉总量HK检测试剂盒 MEGAZYME 淀粉总量HK检测试剂盒 K-TSHK

英文名: Total Starch HK Assay Kit

货号:K-TSHK

规格:100 assays per kit

市场价: 4208

A modification of AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

UV-method for the determination of Total Starch in grains,
animal feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: 100 assays
Method: Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard
Method No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

 

 

抗性淀粉检测试剂盒 K-RSTAR Resistant Starch Assay Kit

特色

抗性淀粉检测试剂盒

英文名:Resistant Starch Assay Kit

货号:K-RSTAR

规格:100 assays per kit

市场价: 3472

The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.

Colourimetric method for the determination of Resistant Starch
in cereal products and feeds

Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose +
maltose

(3) Dissolution of resistant starch pellet in KOH and neutralisation

(α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H2O → D-glucose

(glucose oxidase)
(5) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 120 min (plus overnight incubation)
Detection limit: 2-100% of sample weight
Application examples:
Plant materials, starch samples and other materials
Method recognition:
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Measures enzyme resistant starch
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

 

膳食纤维总量检测试剂盒 K-TDFR-200A Total Dietary Fibre Assay Kit

特色

膳食纤维总量检测试剂盒

英文名:Total Dietary Fibre Assay Kit

货号:K-TDFR-200A

规格:200 assays per kit

市场价: 4736

分析物意义:小肠不能消化的碳水化合物

Megazyme检测试剂盒优点:试剂稳定。成本低。AOAC方法985.29和991.43;AACC方法32-07和32-21

The Total Dietary Fiber test kit is suitable is suitable for the measurement and analysis of Total Dietary Fiber.

For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials

Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol
precipitation

(4) Ash and residual protein determined on DF residues
and subtracted

Kit size: 100 / 200 assays
Method: Hydrolysis / removal of non-dietary
fibre components
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:
AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)

Total Dietary Fiber Assay Kit, for the measurement and analysis of total, soluble and insoluble dietary fiber according to AOAC and AACC approved methods. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our booklet are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.

2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.

3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

Two separate methods are described in the associated data booklet:

METHOD 1:

DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER

Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

METHOD 2:

DETERMINATION OF TOTAL DIETARY FIBER

Based on AACC method 32-05.01 and AOAC Method 985.29.

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years
  • High purity / standardised enzymes employed
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format

 

 

重组人血清白蛋白,rHSA


重组人血清白蛋白,rHSA

货号:188267
包装:50ml

  • 英文名:Recombinant Human Serum Albumin
  • 级别:细胞培养
  • 分子量:66.5kDa
  • 品牌
  • CAS:70024-90-7
  • 外观:略带粘性,黄色或棕色透明液体
  • 产品详情

    人血清白蛋白(Human Serum Albumin,HSA)作为人血浆中含量最丰富的蛋白,是激素、脂类等物质的转运载体,其主要生理功能是调节血浆pH值和维持血浆渗透压。
    金畔生物提供的rHSA(Recombinant Human Serum Albumin),是利用水稻胚乳细胞表达平台和纯化平台研发的重组人血清白蛋白,不含有动物源成分,杜绝血液源的病毒感染风险。
    重组人血清白蛋白,rHSA
    与胎牛血清(FBS)、血浆来源白蛋白(pHSA)和牛血清白蛋白(BSA)相比,rHSA具有更高的纯度,更好的批次稳定性,可以用于各类研究,包括生物制药、细胞治疗、基因治疗的细胞培养等,可替换血清,促进细胞生长。同时,rHSA也被广泛用于生物医药生产中作为药物载体,疫苗保护剂、细胞冻存保护剂和医疗器械包埋剂等。咨询订购热线:021-50837765
    理化性质
    pHSA
    rHSA
    氨基酸序列
    一致
    N-端氨基酸
    DAHKSEV
    DAHKSEV
    C-端氨基酸
    KLVAASQAALGL
    KLVAASQAALGL
    糖苷修饰
    分子量 (MALDl)
    66.554 (kDa)
    66.550 (kDa)
    等电点 (pl)
    4.8
    4.8
    药物结合活性
    相近
    热稳定性
    熔点 65℃
    熔点 65℃
    酯酶活性
    相同
    晶体结构
    相同
    产品参数:
    货号:188267
    规格:50ml,20%浓度 或 10g
    来源:水稻种子
    外观:略带粘性,黄色或棕色透明液体
    浓度:20%,10g/50mL/瓶
    纯度:99%
    pH值:6.4-7.4
    分子量:66.5kDa
    内毒素:<1.67EU/ml
    CAS No:70024-90-7 
    应用领域: 

    生物制药、人用疫苗、细胞培养、细胞存储、化药分子载体、医疗器械、体外诊断等 

    StemSure(R) 细胞冻存液|195-16031


    StemSure(R) 细胞冻存液

    货号:195-16031
    包装:100 ml

  • 英文名:StemSure(R) Freezing Medium
  • 级别:for Cell Culture
  • 品牌:wako
  • StemSure(R) 细胞冻存液(货号:195-16031),为细胞用无血清冻存液。可用于包括小鼠胚胎干细胞在内的多种细胞的冷冻保存。

    产品详情质检信息

    StemSure(R) 细胞冻存液 195-16031

    细胞培养用,100mL

    StemSure® 细胞冻存液(货号:195-16031),为细胞用无血清冻存液。可用于包括小鼠胚胎干细胞在内的多种细胞的冷冻保存。含有10%的二甲基亚砜。
    生产厂商:富士-和光化学纯药株式会社
    外观:Yellow – yellowish red, liquid
    纯度:细菌内毒素<=30.0EU/mL
    储存条件:保持在2-10摄氏度。
    GHS:警告
    参考文章
    Wako人多能性干细胞无血清培养基 StemSure(R) hPSC培养基Δ

    Y-27632 MF


    Y-27632 MF

    货号:259-00613
    包装:5 mg

  • 英文名:Y-27632 MF
  • 级别:for Cell Culture
  • 分子式:C14H21N3O?2HCl.H2O
  • 分子量:338.27
  • 品牌:wako
  • CAS:331752-47-7
  • 外观:白色至浅黄色,结晶性粉末至粉末
  • 产品详情质检信息

    纯度:98.0+% (HPLC)
       等级:
    for Cellbiology
    一种选择性和有效的ROCK(Rho相关的卷曲螺旋形成激酶)抑制剂。Y-27632具有多种作用,例如通过ROCK信号转导系统收缩血管平滑肌。
    (Ki = 140nmol / L p160ROCK)据报道,在人ES细胞和人iPS细胞的细胞分散过程中,细胞死亡受到抑制,并且冷冻保存后的细胞活力得到改善。

    外貌 白色至浅黄色,结晶性粉末至粉末
    溶解度 它可溶于水和乙醇,几乎不溶于丙酮。
    沸点 约85℃
    比旋光度 [α]D20+2.0至+10.0°(c=1.0,CH3OH)
    纯度 内毒素:<0.25EU/mg

    StemSure hPSC Medium Δ StemSure人多能干细胞培养基


    StemSure(R) 人多能干细胞培养基

    货号:197-17571
    包装:100 ml

  • 英文名:StemSure hPSC Medium Δ StemSure人多能干细胞培养基
  • 级别:for Cell Culture
  • 品牌:wako
  • 外观:红色,澄清液体
  • 产品详情质检信息

    StemSure(R) 人多能干细胞培养基 197-17571

    细胞培养用,100mL

    StemSure(R) 人多能干细胞培养基(货号:197-17571),适用于在无饲养层细胞、无血清和无动物组分环境下维持人多能干细胞(hPSCs)、人ES细胞和人iPS细胞的未分化能力。已有大量实验证明,StemSure® hPSC MediumΔ 能维持多种细胞系(201B7,253G4,Tic,WA01) 的未分化能力。
    生产厂商:富士-和光化学纯药株式会社
    外观:红色,澄清液体
    PH=7.0-7.2
    细菌内毒素:1.0EU/ml
    储存条件:保持在2-10摄氏度。
    该培养基蛋白质含量低,且不含白蛋白。
    利用该培养基,细胞可以在多种细胞外基质上培养,在传代过程中添加Y-27632也可以进行单细胞传代。
    本产品仅供研究使用。勿用于人体。
     
    货号
    品名
    包装规格
    197-17571
    StemSure® hPSC Medium Δ
    100mL
    193-17573
    StemSure® 人多能干细胞培养基
    100mLx4
    参考文章
    Wako人多能性干细胞无血清培养基 StemSure(R) hPSC培养基Δ

    (4-氯苯巯基)(10-甲基-9,10-二氢化吖啶亚甲基)磷酸二钠盐


    (4-氯苯巯基)(10-甲基-9,10-二氢化吖啶亚甲基)磷酸二钠盐

    货号:ECD0532A
    包装:mg

  • 英文名:APS-5
  • 分子式:C21H15ClNNa2O4PS
  • 分子量:489.82
  • 品牌
  • CAS:193884-53-6
  • 外观:类白色固体粉末
  • 产品详情质检信息

    反应机理
    (4-氯苯巯基)(10-甲基-9,10-二氢化吖啶亚甲基)磷酸二钠盐
      APS-5与碱性磷酸酶(ALP)混合后,碱性磷酸酶磷酸根水解后,底物立即发生分解反应释放出光子,在特定的碱性磷酸酶浓度范围内,释放的光子数量与溶液中碱性磷酸酶的浓度成正比,所以可以用于碱性磷酸酶的定量检测。
    产品优势
      1、高灵敏度-能达到10的-19次方摩尔浓度;
      2、发光强度高-在短时间内可以达到发光峰值(10s内);
      3、持续稳定发光-其发光过程在25-35℃内不受温度影响,不需温度控制.
      4、碱性磷酸酶的浓度与释放的光子数量有良好的线性关系
      5、空白发光值低
    应用领域
     

      1、化学发光平台(碱性磷酸酶体系)
      2、碱性磷酸酶定量检测
      3、作为发光探针用于基因芯片

    相关产品
    藻红蛋白(R-Phycoerythrin,R-PE)—新型荧光标记染料
    别藻蓝蛋白(Allophycocyanin, APC)——超敏荧光染料
    进口品质 Jackson二抗之链霉亲和素、链霉亲和素标记物系列
    金畔生物可提供各种荧光染料,如藻红蛋白(R-PE)、菁类染料(Cy系列)等,以及各类荧光标记物,均可定制大包装,详情咨询金畔生物021-50837765!或者联系我司诊断团队。

    Y-27632 MF


    Y-27632 MF

    货号:257-00511
    包装:1 mg

  • 英文名:Y-27632 MF
  • 分子式:C14H21N3O?2HCl?H2O
  • 分子量:338.27
  • 品牌:wako
  • CAS:331752-47-7
  • 外观:白色至浅黄色,结晶性粉末至粉末
  • 产品详情质检信息

    纯度:98.0+% (HPLC)
       等级:
    for Cellbiology
    一种选择性和有效的ROCK(Rho相关的卷曲螺旋形成激酶)抑制剂。Y-27632具有多种作用,例如通过ROCK信号转导系统收缩血管平滑肌。
    (Ki = 140nmol / L p160ROCK)据报道,在人ES细胞和人iPS细胞的细胞分散过程中,细胞死亡受到抑制,并且冷冻保存后的细胞活力得到改善。

    外貌 白色至浅黄色,结晶性粉末至粉末
    溶解度 它可溶于水和乙醇,几乎不溶于丙酮。
    沸点 约85℃
    比旋光度 [α]D20+2.0至+10.0°(c=1.0,CH3OH)
    纯度 内毒素:<0.25EU/mg

    StemSure Freezing Medium StemSure细胞冻存液


    StemSure Freezing Medium StemSure细胞冻存液

    货号:195-16031
    包装:100 ml

  • 英文名:StemSure Freezing Medium StemSure细胞冻存液
  • 品牌:wako
  • 产品详情质检信息

     感谢您的关注,该商品暂无详细信息,请联系业务人员咨询 021-50837765,或咨询我们的在线客服。

    Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003)|链霉亲和素及标记物


    Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003)

    货号:115-035-003

  • 英文名:Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L)
  • 宿主:Goat
  • 反应种属:Mouse
  • 特异性:IgG (H+L)
  • 标记:Horseradish Peroxidase
  • 抗体形式:Whole IgG
  • 克隆性:Polyclonal
  • 产品详情质检信息

    Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)

    货号:115-035-003
    包装:2.0 ml
    反应种属:小鼠
    宿主:山羊
    抗体形式:Whole IgG
    特异性:IgG (H+L)
    标记:辣根过氧化物酶 HRP
    克隆性:多克隆
    RRID: AB_10015289
    说明
    用免疫亲和层析法从抗血清中分离出完整的IgG抗体,其中Fc片段和两个抗原结合的Fab片段通过二硫键连接,因此是二价的。平均分子量的报导值约为160kda。完整IgG形式的抗体,适用于大多数免疫检测过程,且最具成本效益。
    在进行免疫电泳和/或ELISA检测时,该抗体与小鼠IgG完整分子反应。它还与其他小鼠免疫球蛋白轻链发生反应。未检测到对抗非免疫球蛋白类血清蛋白的抗体存在。该抗体可能与其他物种的免疫球蛋白发生交叉反应。
    物理状态:冻干固体
    存贮与复溶:冻干固体在2-8℃下保存。使用所示体积的去离子水复溶,如果溶液不透明进行离心。使用当天制备工作稀释液。在2-8℃下,产品作为未稀释液体,可以保持稳定6周左右。
    复溶后长期存贮:等分并在-70℃或更低温度下冷冻。避免反复冻融。按顺序加入同等体积的乙二醇(ACS级或更好的)以制备最终浓度为50%的溶液,并在-20℃下作为液体保存。
    效期:自复溶之日起的一年内。如果复测结果允许用于预期用途,则效期可适当延长。
    纯度:通过免疫亲和层析法,使用抗原结合琼脂糖磁珠,从抗血清中纯化抗体。
    缓冲液:0.01M磷酸钠,0.25M 氯化钠,pH 7.6
    稳定剂:15mg/ml牛血清白蛋白(不含IgG,不含蛋白酶)
    防腐剂:未添加(警示:使用叠氮钠作为防腐剂,会大大抑制辣根过氧化物酶的活性。)
    建议工作浓度或稀释范围
    免疫组织/细胞化学,1:500-1:5000
    ELISA和带显色底物的免疫印迹法WB,1:5000-1:100000
    带ECL底物的免疫印迹法,1:10000-1:200000
    由于最佳稀释度函数涉及多个因数,如抗原密度、渗透性等,所以以范围来表征稀释因子。实际使用的稀释度须依经验而定。
    标记物
    辣根过氧化物酶(HRP)标记物是通过改良的Nakane和Kawaoi方法制备(J. Histochem. Cytochem. 1974. 22, 1084)。 过氧化物酶标记物,通常用于免疫组织化学、免疫印迹(WB)和酶联免疫法(ELISA)。亲和纯化的抗辣根过氧化物酶及其标记物,可用于辣根过氧化物酶抗原的检测或含HRP试剂的信号放大。对于哺乳动物细胞的免疫染色,使用抗辣根过氧化物酶的一个优点是减少背景干扰,因为抗体不能识别在细胞中存在的类似内源性过氧化物酶的酶类。
     

    MCI缓冲液L-8500-PH套装 (608-07461)


    MCI缓冲液L-8500-PH套装 (608-07461)

    货号:608-07461
    包装:1 Kit

    MCI缓冲液L-8500-PH套装(608-07461)系柠檬酸钠类缓冲液,适合日立L-8800、L-8500A和L-8500型高速氨基酸分析仪使用。用于蛋白质水解产物的氨基酸分析。

    产品详情质检信息

    产品信息
    货号
    608-07461
    产品名称
    MCITM缓冲溶液套装 L-8500-PH-KIT
    品牌
    MCI
    制造商
    三菱化学
    制造商货号
    PHK
    规格
    氨基酸分析
    包装
    1 Kit
    内含
    PH1
    L-8500-PH-1
    1L×2
    第1缓冲溶液
    PH2
    L-8500-PH-2
    1L×1
    第2缓冲溶液
    PH3
    L-8500-PH-3
    1L×1
    第3缓冲溶液
    PH4
    L-8500-PH-4
    1L×2
    第4缓冲溶液
    PHRG
    L-8500-PH-RG
    1L×1
    再生液
    用途
    PH型,系柠檬酸钠类缓冲液,适合日立L-8800,L-8500A和L-8500型高速氨基酸分析仪使用,用于蛋白质水解产物的氨基酸分析
    注意
    1)室温、避光保存,避免接触氨
    2)缓冲溶液组分与日立L-8500型高速氨基酸分析仪完全匹配,无需稀释或微调pH值
    3)用于日立L-8500A型高速氨基酸分析仪时,请调节加载于缓冲溶液容器的氮气压力,控制在0.35-0.4kgf/cm2,或0.034-0.03MPa。
    4)请在盒体上标明的有效期之前使用。一旦瓶口打开,溶液质量可能发生变化,请一次性加完,即开即用。
    5)所有套装包含充足的缓冲溶液,允许日立L-8800型,L-8500A型和L-8500型高速氨基酸分析仪在标准操作条件下进行20天左右的连续操作。
    6)关于具体操作,请参考日立L-8800型,L-8500A型和L-8500型高速氨基酸分析仪产品说明书。
    7)本产品仅供研究用途,请勿应用于人体。
    危险性质
    危险性质
    UN: Class 9 – Misc.
    保存
    2-10 ℃
    价格信息
    价格
    RMB 4700.00
    货期
    2-4周
    相关产品
    605-07471
    MCI Buffer L-8500-PF1)  Kit
    608-07461
    MCI Buffer L-8500-PH2)  Kit
    注:
    Note1): PF 用于生物体液分析
    Note2): PH 用于蛋白质水解产物分析
    参考文章
    1. 日立氨基酸分析仪配套试剂解决方案
    2. 我国食品行业上海金畔生物科技有限公司是日本和光纯药(Wako)的授权一级代理。(点击此处查看代理资格)所有Wako以及和光经销的试剂产品,原装进口,质量可靠,价格合理,每周五下单,货期稳定。欢迎新老客户来电垂询订购。全国统一服务热线:021-50837765。