D-果糖/D-葡萄糖检测试剂盒 , D-Fructose plus D-glucose assay kit , 货号：K-FRUGL
品牌：Megazyme
货号：K-GLUGL
中文品名：D-果糖/D-葡萄糖检测试剂盒
品名：D-Fructose plus D-glucose assay kit
规格：110 次检测 (手工) / 1100 (微孔板) / 1100 (自动分析仪)
应用：D-果糖/D-葡萄糖检测试剂盒是一种酶法-紫外检测方法，用于测量和检测植物和食品中D-果糖,和/或D-葡萄糖含量。辅助因子更稳定，溶解后在4°C下可保存超过1年。适合手工、微孔板和自动分析仪分析。
原理：
检测方法：分光光度法 @340 nm
反应时间：~13min
检测限：0.66 mg/L
应用案例：
葡萄酒、啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、食疗食品、面包、焙烘食品、糖果、甜点、糕点、冰淇淋、水果蔬菜、调味品、烟草、化妆品、药品、造纸和其他原料(如生物培养、样品等)
方法识别：
基于上述原理的检测方法已经被AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK和IOCCC等组织接受。
试剂盒组成：
| Bottle 1: | 缓冲溶液(15 mL, pH 7.5) 添加叠氮钠(0.02%)作为防腐剂 |
| 4°C下稳定超过 2年 | |
| Bottle 2: | NADP+, ATP和PVP。 |
| -20°C稳定超过 5年 | |
| Bottle 3: | 己糖激酶和葡萄糖-6-磷酸脱氢酶悬浮液, 2.25mL. |
| 4°C下稳定超过 2年 | |
| Bottle 4: | 磷酸葡萄糖异构酶悬浮液(2.25 mL)。 |
| 4°C下稳定超过 2年 | |
| Bottle 5: | D-葡萄糖和D-果糖标准溶液(5 mL, 每种糖浓度为0.2 mg/mL)。 |
| 4°C下稳定超过 2年 |
优点：
包含PVP可预防单宁抑制
经过法国Suze la Rousse葡萄酒大学验证
价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年（针对手工分析应用）
在25°C或37°C下都能快速检测 官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
辅助因子更稳定
适合手工、微孔板和自动分析仪分析。
经过法国Suze la Rousse葡萄酒大学验证
价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年（针对手工分析应用）
在25°C或37°C下都能快速检测 官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
辅助因子更稳定
适合手工、微孔板和自动分析仪分析。
参考文献：
Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.
Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.
Lactose fermentation by Kombucha – a process to obtain new milk–based beverages. Ili?i?, M., Kanuri?, K., Milanovi?, S., Lon?ar, E., Djuri?, M. & Malbaša, R. (2012). Romanian Biotechnological Letters, 17(1), 7013-7021.
Sourdough-leavened bread improves postprandial glucose and insulin plasma levels in subjects with impaired glucose tolerance. Maioli, M., Pes, G. M., Sanna, M., Cherchi, S., Dettori, M., Manca, E. & Farris, G. A. (2008). Acta Diabetologica, 45(2), 91-96.
Endocrine and metabolic effects of consuming fructose- and glucose-sweetened beverages with meals in obese men and women: Influence of insulin resistance on plasma triglyceride responses. Teff, K. L., Grudziak, J., Townsend, R. R., Dunn, T. N., Grant, R. W., Adams, S. H., Keim, N. L., Cummings, B. P., Stanhope, K. L. & Havel, P. J. (2009). Journal of Clinical Endocrinology & Metabolism, 94(5), 1562-1569.
Metabolic and endocrine profiles in response to systemic infusion of fructose and glucose in rhesus macaques. Adams, S. H., Stanhope, K. L., Grant, R. W., Cummings, B. P. & Havel, P. J. (2008). Endocrinology, 149(6), 3002-3008.
β-Fructofuranosidase and sucrose phosphorylase of rumen bacterium Pseudobutyrivibrio ruminis strain 3. Kasperowicz, A., Stan-Glasek, K., Guczynska, W., Pristas, P., Javorsky, P., Vandzurova, A. & Michalowski, T. (2012). World Journal of Microbiology and Biotechnology, 28(3), 1271-1279.
Comparative effects of fructose and glucose on lipogenic gene expression and intermediary metabolism in HepG2 liver cells. Hirahatake, K. M., Meissen, J. K., Fiehn, O. & Adams, S. H. (2011). PloS one, 6(11), e26583.
Measurement of glucose and fructose in clinical samples using gas chromatography/mass spectrometry. Wahjudi, P. N., Patterson, M. E., Lim, S., Yee, J. K., Mao, C. S. & Lee, W. N. P. (2010). Clinical Biochemistry, 43(1-2), 198-207.
Physiological studies of Leuconostoc mesenteroides strain NRRL B-1149 during cultivation on glucose and fructose media. Bivolarski, V., Vasileva, T., Shukla, R., Goyal, A. & Iliev, I. (2012). Journal of BioScience & Biotechnology, 1(3), 235-240.
Aldose reductase is implicated in high glucose‐induced oxidative stress in mouse embryonic neural stem cells. Fu, J., Tay, S. S. W., Ling, E. A. & Dheen, S. T. (2007). Journal of Neurochemistry, 103(4), 1654-1665.
Immobilised yeast grape must deacidification in a recycle fixed bed reactor. Portugal, I., Ribeiro, S. C., Xavier, A. M. R. B., Centeno, F. & Strehaiano, P. (2011). International Journal of Food Science & Technology, 46(2), 284-289.
The Elemental Composition of Synechocystis sp. PCC6803 under Nutrient Limiting Conditions. Blom, M. (2014). University of Amsterdam, Bachelor Thesis.
使用手册：
K-FRUGL使用手册
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