D-葡萄糖[GOPOD法]检测试剂盒, D-Glucose assay kit(GOPOD-Format), 货号：K-GLUC
品牌：Megazyme
货号：K-GLUC
中文品名：D-葡萄糖[GOPOD法]检测试剂盒
品名：D-Glucose assay kit(GOPOD-Format)
规格：660次/盒
应用：D-葡萄糖[GOPOD法]检测试剂盒包含用于检测和分析谷物提取物中D-葡萄糖含量的高纯试剂，也可用于其他Megazyme检测试剂盒的联检。
原理：
比色法测定食品、饮料和其它配料中D-葡萄糖含量
(glucose oxidase)
(1) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → quinoneimine + 4H2O
检测方法：分光光度法 @510 nm
反应时间：~20min
检测限：100mg/L
应用案例：
葡萄酒、啤酒、果汁、软饮料、牛奶、果酱、食疗食品、烘焙食品、糖果、水果、蔬菜、烟草、化妆品、药品、饲料、纸和其他食品原料 (例如 生物培基、样品等）。
方法识别：
在临床化学和食品分析中广泛使用和接受
试剂盒组成：
| Bottle 1: (x 2) | GOPOD试剂缓冲液。缓冲液(50 mL, pH 7.4), 对羟基苯甲酸和叠氮钠(0.095% w/v). |
| 稳定超过 2年 at 4°C | |
| Bottle 2: (x 2) | GOPOD试剂酶。优点：
所有试剂的保质期大于生产日期后12个月
价格非常具有竞争力 检测方法十分简单 包含标准溶液 参考文献：
Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.
Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.
Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris. Choix. F. J., Bashan, Y., Mendoza, A. & de-Bashan, L. E. (2014). Journal of Biotechnology, 177, 22-34.
In vitro hypoglycemic effects of different insoluble fiber-rich fractions prepared from the peel of Citrus sinensis L. cv. Liucheng Chau, C. F., Huang, Y. L. & Lee, M. H. (2003). Journal of Agricultural and Food Chemistry, 51(22), 6623-6626.
Dietary fibers from mushroom sclerotia: 3. In vitro fermentability using human fecal microflora. Wong, K. H., Wong, K. Y., Kwan, H. S. & Cheung, P. C. K. (2005). Journal of Agricultural and Food Chemistry, 53(24), 9407-9412.
Potential hypoglycaemic effects of insoluble fibres isolated from foxtail millets [Setaria italica (L.) P. Beauvois]. Bangoura, M. L., Nsor‐Atindana, J., Zhu, K., Tolno, M. B., Zhou, H. & Wei, P. (2013). International Journal of Food Science & Technology, 48(3), 496-502.
使用手册：
K-GLUC使用手册
相关产品：
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D-Fructose/D-Glucose Assay Kit
Starch Damage Assay Kit
Lactose/Sucrose/D-Glucose Assay Kit
Maltose/Sucrose/D-Glucose Assay Kit
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