总膳食纤维检测试剂盒, Inegrated Total Dietary Fibre Assay Kit , 货号：K-INTDF
品牌：Megazyme
货号：K-INTDF
中文品名：总膳食纤维检测试剂盒
品名：Inegrated Total Dietary Fibre Assay Kit
规格：100 次检测
应用：总膳食纤维检测试剂盒，用于总膳食纤维特定检测和分析。
K-INTDF 是总膳食纤维测定（包括抗性淀粉和非消化寡糖）的一个完整过程（AOAC方法2009.01和2011.25）。该方法结合了AOAC官方分析方法2002.02、985.29、991.43，2001.03的关键指标。
特定膳食纤维组分测定如下：
1、不溶性膳食纤维（IDF），高分子量的水溶性膳食纤维（SJZFP）和低分子量可溶性膳食纤维（SDFS）测定（AOAC方法2011.25）
2、总膳食纤维：高分子量膳食纤维（hmwdf）和低分子量可溶性膳食纤维（SDFS）的测定（AOAC方法2009.01）
这些方法中所使用的酶纯度很高；不含对β-葡聚糖、果胶和木聚糖具有活性的酶。
K-INTDF 是总膳食纤维测定（包括抗性淀粉和非消化寡糖）的一个完整过程（AOAC方法2009.01和2011.25）。该方法结合了AOAC官方分析方法2002.02、985.29、991.43，2001.03的关键指标。
特定膳食纤维组分测定如下：
1、不溶性膳食纤维（IDF），高分子量的水溶性膳食纤维（SJZFP）和低分子量可溶性膳食纤维（SDFS）测定（AOAC方法2011.25）
2、总膳食纤维：高分子量膳食纤维（hmwdf）和低分子量可溶性膳食纤维（SDFS）的测定（AOAC方法2009.01）
这些方法中所使用的酶纯度很高；不含对β-葡聚糖、果胶和木聚糖具有活性的酶。
原理：
谷物、食品、饲料及其他材料中总膳食纤维的测定。 CODEX 一类方法(2011)
检测方法： 水解/去除非膳食纤维组分
反应时间：~ 累计3小时的工作量(跨2个工作日)
检测限：样品重量的0.5-100%
应用案例：
食品配料、食品和其它食材。
方法识别：
该检测方法已经被AOAC (Methods 2009.01 和 2011.25), AACC (Method 32-45.01 和 32-50.01) 和CODEX (一类方法)收录认可。
试剂盒组成：
| Bottle 1: | 浓缩的胰腺α-淀粉酶(货号：E-PANAA); 2 g, 150,000 Ceralpha Units/g. |
| -20°C下稳定超过 5年 | |
| Bottle 2: | 淀粉葡萄糖苷酶 (货号：E-AMGDF) (20 mL, 3300 Units/mL) |
| 4°C稳定超过3年 | |
| Bottle 3: | 纯化的蛋白酶 (货号：E-BSPRT) (10 mL, 350 酪氨酸 units/mL). |
| -20°C下稳定超过 5年 | |
| Bottle 4: | 液相保留时间标准品 [麦芽糊精与麦芽糖(4:1比例), 约5 g] |
| 室温干燥下保存稳定超过 3年 | |
| Bottle 5: | D-山梨醇 (约12 g, 干粉) |
| 室温干燥下保存稳定超过 3年 | |
| Bottle 6: | D-葡萄糖加D-果糖标准溶液 (5 mL, 每个糖组分0.2 mg/mL). |
| 4°C下稳定超过 2年 |
优点：
唯一符合国际食品法典委员会膳食纤维定义的方法
使用高纯度、标准化的酶
所有试剂制备后可以稳定保存超过2年
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
价格非常具有竞争力
使用高纯度、标准化的酶
所有试剂制备后可以稳定保存超过2年
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
价格非常具有竞争力
参考文献：
Determination of total dietary fibre and available carbohydrates: A rapid integrated procedure that simulates in vivo digestion.McCleary, B. V., Sloane, N. & Draga, A. (2015). Starch/Stärke, 67(9-10), 860–883.
Measurement of dietary fibre components: the importance of enzyme purity, activity and specificity. McCleary, B. V. (2001), “Advanced Dietary Fibre Technology”, (B. V. McCleary and L. Prosky, Eds.), Blackwell Science, Oxford, U.K., pp. 89-105.
Dietary fiber and available carbohydrates. McCleary, B. V. & Rossiter, P. C. (2007). “Dietary Fiber: An International Perspective for Harmonization of Health Benefits and Energy Values”, (Dennis T. Gordon and Toshinao Goda, Eds.), AACC International, Inc., pp. 31-59.
Measuring dietary fibre. McCleary, B. V. (1999). The World of Ingredients, 50-53.
Enzyme purity and activity in fibre determinations. McCleary, B. V. (1999). Cereal Foods World, 44(8), 590-596.
Importance of enzyme purity and activity in the measurement of total dietary fibre and dietary fibre components. McCleary, B. V. (2000). Journal of AOAC International, 83(4), 997-1005.
Two issues in dietary fiber measurement. McCleary, B. V. (2001). Cereal Foods World, 46(4), 164-165.
Dietary fibre analysis. McCleary, B. V. (2003). Proceedings of the Nutrition Society, 62, 3-9.
Measurement of novel dietary fibres. McCleary, B. V. & Rossiter, P. (2004). Journal of AOAC International, 87(3), 707-717.
An integrated procedure for the measurement of total dietary fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates. McCleary, B. V. (2007). Analytical and Bioanalytical Chemistry, 389(1), 291-308.
Development and evaluation of an integrated method for the measurement of total dietary fibre. McCleary, B. V., Mills, C. & Draga, A. (2009). Quality Assurance and Safety of Crops & Foods, 1(4), 213–224.
Determination of total dietary fiber (CODEX definition) by enzymatic-gravimetric method and liquid chromatography: collaborative study. McCleary, B. V., DeVries, J. W., Rader, J. I., Cohen, G., Prosky, L., Mugford, D. C., Champ, M. & Okuma, K. (2010). Journal of AOAC International, 93(1), 221-233.
Measurement of total dietary fiber using AOAC method 2009.01 (AACC International approved method 32-45.01): Evaluation and updates. McCleary, B. V., Sloane, N., Draga, A. & Lazewska, I. (2013). Cereal Chemistry, 90(4), 396-414.
Modification to AOAC Official Methods 2009.01 and 2011.25 to allow for minor overestimation of low molecular weight soluble dietary fiber in samples containing starch. McCleary, B. V. (2014). Journal of AOAC International, 97(3), 896-901.
Dietary fibre fractions in cereal foods measured by a new integrated AOAC method. Hollmann, J., Themeier, H., Neese, U. & Lindhauer, M. G. (2013). Food chemistry, 140(3), 586-589.
Adaptation of the AOAC 2011.25 Integrated Total Dietary Fiber Assay to determine the dietary fiber and oligosaccharide content of dry edible beans. Kleintop, A. E., Echeverria, D., Brick, L. A., Thompson, H. J. & Brick, M. A. (2013). Journal of Agricultural and Food Chemistry, 61(40), 9719-9726.
使用手册：
K-INTDF使用手册
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