Megazyme 半乳甘露聚糖检测试剂盒(K-GALM)

特色

 

半乳甘露聚糖检测试剂盒

货号:K-GALM
包装:1 kit

 

  • 英文名:Galactomannan Assay Kit
  • 品牌:Megazyme

</

半乳甘露聚糖检测试剂盒,Galactomannan Assay Kit, 货号:K-GALM,100 次检测,适于食品和植物产品中办乳甘露聚糖的检测和分析。

产品详情

半乳甘露聚糖检测试剂盒, Galactomannan Assay Kit, 货号:K-GALM
品牌:Megazyme
货号:K-GALM
中文品名:半乳甘露聚糖检测试剂盒
品名:Galactomannan Assay Kit
规格:100 次检测
应用:半乳甘露聚糖检测试剂盒适于食品和植物产品中办乳甘露聚糖的检测和分析。
原理:
Megazyme 半乳甘露聚糖检测试剂盒(K-GALM)
检测方法:分光光度法 @340 nm
反应时间:~90min
检测限:样品重量的1-100%
应用案例:
种子,碾磨粉和食品配料
方法识别:
新方法
试剂盒组成:
Bottle 1: 缓冲溶液(25 mL, pH 8.6) 添加叠氮钠(0.02%)作为防腐剂(缓冲液A)
4°C下稳定超过 2年
Bottle 2: NAD+
-20°C稳定超过 5年
Bottle 3: β-甘露聚糖酶悬浮液(A. niger; 1.1 mL)
4°C下稳定超过 4年
Bottle 4: β-半乳糖苷酶(瓜尔豆胶种子)加上β-甘露聚糖酶悬浮液,2.2 mL。
4°C下稳定超过 4年
Bottle 5: β-半乳糖脱氢酶 2.4 mL。
4°C下稳定超过 2年
Bottle 6: D-半乳糖标准溶液 (5 mL, 0.4 mg/mL 溶于0.02%叠氮钠溶液中)。
4°C下稳定超过 4年
Bottle 7: 长豆角半乳糖甘露聚糖粉质控品(半乳甘露聚糖含量在小瓶标签上标明)
室温下稳定超过 5年

优点:

试剂盒内包含半乳糖脱氢酶
价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年
仅提供酶试剂盒
操作简单
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
适合手工、微孔板和自动分析仪分析。
参考文献:
An enzymic technique for the quantitation of galactomannan in guar Seeds. McCleary, B. V. (1981). Lebensmittel-Wissenschaft & Technologie, 14, 56-59.
Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
Metabolic and genetic perturbations accompany the modification of galactomannan in seeds of Medicago truncatula expressing mannan synthase from guar (Cyamopsis tetragonoloba L.). Naoumkina, M., Vaghchhipawala, S., Tang, Y., Ben, Y., Powell, R. J. & Dixon, R. A. (2008). Plant Biotechnology Journal, 6(6), 619-631.
Enzymatic improvement of guar‐based thickener for better‐quality silk screen printing. Baldaro, E., Gallucci, M., Formantici, C., Issi, L., Cheroni, S. & Galante, Y. M. (2012). Coloration Technology, 128(4), 315-322.
Nutrient utilisation and intestinal fermentation are differentially affected by the consumption of resistant starch varieties and conventional fibres in pigs. Rideout, T. C., Liu, Q., Wood, P. & Fan, M. Z. (2008). British Journal of Nutrition, 99(05), 984-992.
使用手册:
K-GALM使用手册
相关产品:
Galactomannan (Guar; Medium Viscosity)
Galactomannan (Carob; Low Viscosity)
Galactomannan (Carob; High Viscosity)
Galactomannan (Guar; High Viscosity)
Galactomannan (Guar; High Visc, Gal depleted; 21% Gal)
Galactomannan (Guar; High Visc, Gal depleted; 28% Gal)
endo-1,4 β-Mannanase (Bacillus sp.)

MEGAZYME 淀粉总量HK检测试剂盒 K-TSHK

特色

Megazyme中文站-经销爱尔兰MEGAZYME试剂盒、酶标准品、糖酶片剂

淀粉总量HK检测试剂盒 MEGAZYME 淀粉总量HK检测试剂盒 K-TSHK

英文名: Total Starch HK Assay Kit

货号:K-TSHK

规格:100 assays per kit

市场价: 4208

A modification of AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

UV-method for the determination of Total Starch in grains,
animal feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: 100 assays
Method: Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard
Method No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included